Author: Ahat, Erpan; Xiang, Yi; Zhang, Xiaoyan; Bekier, Michael E.; Wang, Yanzhuang
Title: GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of a5ß1 integrin Document date: 2019_3_15
ID: rfs7m6or_39
Snippet: For restoration of the α5β1-integrin level in GRASP-KO cells, cells at ∼60% confluency in a 6-cm plate were transfected with 8 µg of α5-GFP cDNA construct by Lipofectamine 2000 (Invitrogen, cat. no. 11668019) following the manufacture's instructions, and pEGFP-N1 was used as a control. In three independent experiments, the transfection rate of α5-GFP in GRASP55, GRSAP65, and double-knockout cells was 66 ± 1%, 67 ± 3%, and 71 ± 1%, respe.....
Document: For restoration of the α5β1-integrin level in GRASP-KO cells, cells at ∼60% confluency in a 6-cm plate were transfected with 8 µg of α5-GFP cDNA construct by Lipofectamine 2000 (Invitrogen, cat. no. 11668019) following the manufacture's instructions, and pEGFP-N1 was used as a control. In three independent experiments, the transfection rate of α5-GFP in GRASP55, GRSAP65, and double-knockout cells was 66 ± 1%, 67 ± 3%, and 71 ± 1%, respectively. This was tested at 72 h posttransfection, a time point used for the cell adhesion and migration assays.
Search related documents:
Co phrase search for related documents- cell adhesion and double knockout: 1, 2
- cell adhesion and double knockout cell: 1
- cell adhesion and GRASP KO cell: 1, 2
- cell adhesion and GRASP KO cell level: 1, 2
- cell adhesion and migration assay: 1, 2, 3, 4, 5, 6
- cell adhesion and migration assay cell adhesion: 1, 2
- cell adhesion and time point: 1, 2
- double knockout and GRASP KO cell: 1, 2
- double knockout and GRASP KO cell level: 1
- double knockout and migration assay: 1
- GRASP KO cell and migration assay: 1
- GRASP KO cell and time point: 1
- independent experiment and time point: 1
Co phrase search for related documents, hyperlinks ordered by date