Author: Yoo, Hyun Jung; Yoon, Sung Soo; Park, Seon Yang; Lee, Eun Young; Lee, Eun Bong; Kim, Ju Han; Song, Yeong Wook
Title: Gene Expression Profile during Chondrogenesis in Human Bone Marrow derived Mesenchymal Stem Cells using a cDNA Microarray Document date: 2011_6_20
ID: unr44dvp_9
Snippet: Flow cytometry was performed to determine MSCs positive for SH2. Cells were permeabilized with ice cold 75% methanol in PBS for 30 min at 4°C, and washed three times. A FITC-conjugated SH2 antibody (DiNonA), diluted 1:500 in PBS, was then added, and cells were incubated for 1 hr at 4°C. Cells were analyzed within 2 hr of staining using a flow cytometer (FACScali-bur, Becton Dickinson, Bedford, MA, USA). A total of 1 × 10 6 cells were collected.....
Document: Flow cytometry was performed to determine MSCs positive for SH2. Cells were permeabilized with ice cold 75% methanol in PBS for 30 min at 4°C, and washed three times. A FITC-conjugated SH2 antibody (DiNonA), diluted 1:500 in PBS, was then added, and cells were incubated for 1 hr at 4°C. Cells were analyzed within 2 hr of staining using a flow cytometer (FACScali-bur, Becton Dickinson, Bedford, MA, USA). A total of 1 × 10 6 cells were collected for each measurement. Negative control samples were stained with an isotype-matched irrelevant mAb.
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