Author: He, Cheng; Kong, Ling; Zhou, Lijuan; Xia, Jing; Wei, Haixia; Liu, Min; Peng, Hongjuan
Title: Host Cell Vimentin Restrains Toxoplasma gondii Invasion and Phosphorylation of Vimentin is Partially Regulated by Interaction with TgROP18 Document date: 2017_9_5
ID: z9mg639h_8
Snippet: Additionally, to generate an RH/GFP strain ectopically expressing GFP, the recombinant plasmid, pBlue-p24-eGFP-DHFR-TS, was linearized with ApaI and then transfected into parasites. Cell lines in which the plasmid was stably integrated were selected using 3μM pyrimethamine and isolated by limiting dilution (data not shown) [28] . Cos7 cells were infected with T. gondii RH strain tachyzoites for different periods of time (as indicated), and then .....
Document: Additionally, to generate an RH/GFP strain ectopically expressing GFP, the recombinant plasmid, pBlue-p24-eGFP-DHFR-TS, was linearized with ApaI and then transfected into parasites. Cell lines in which the plasmid was stably integrated were selected using 3μM pyrimethamine and isolated by limiting dilution (data not shown) [28] . Cos7 cells were infected with T. gondii RH strain tachyzoites for different periods of time (as indicated), and then fixed with paraformaldehyde and permeabilized with Triton X-100. Red, host cell vimentin labeled using mAb anti-vimentin; green, T. gondii tachyzoites labeled with rabbit anti-ROP2.Cell nuclei were stained with DAPI. The rearrangement of vimentin around T. gondii PVs was observed at different time points after infection (arrowheads).
Search related documents:
Co phrase search for related documents- cell line and different time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- cell line and different time point: 1
- cell line and host cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- different time and host cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
- gondii pv and host cell: 1, 2
Co phrase search for related documents, hyperlinks ordered by date