Selected article for: "Bayesian Markov chain and Markov chain"
Author: Li, Na; Ayinmode, Adekunle B.; Zhang, Hongwei; Feng, Yaoyu; Xiao, Lihua
Title: Host-adapted Cryptosporidium and Enterocytozoon bieneusi genotypes in straw-colored fruit bats in Nigeria
  • Document date: 2018_12_4
    • ID: u5fmb8xz_9
    Snippet: All positive secondary PCR products in this study were sequenced in bi-directionally using the Big Dye Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems, Foster City, CA) on an ABI 3130 Genetic Analyzer (Applied Biosystems). Sequences were assembled using ChromasPro 1.5 (http://technelysium.com.au/ChromasPro.html) and compared with reference sequences in NCBI database using ClustalX (http://clustal.org/) to determine the genotypes of path.....
    Document: All positive secondary PCR products in this study were sequenced in bi-directionally using the Big Dye Terminator v3.1 Cycle Sequencing Kits (Applied Biosystems, Foster City, CA) on an ABI 3130 Genetic Analyzer (Applied Biosystems). Sequences were assembled using ChromasPro 1.5 (http://technelysium.com.au/ChromasPro.html) and compared with reference sequences in NCBI database using ClustalX (http://clustal.org/) to determine the genotypes of pathogens of Cryptosporidium spp. and E. bieneusi in fecal specimens. Phylogenetic trees were constructed from the nucleotide sequence alignments using the Bayesian inference and Monte Carlo Markov Chain (MCMC) methods in MrBayes 3.2.6 (http://nbisweden.github.io/MrBayes), with the posterior probability (pp) values calculated by running 1,000,000 generations. Nucleotide sequences generated from the study were submitted to GenBank under accession numbers MK007969-MK007974.

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