Selected article for: "infection multiplicity and MOI infection multiplicity"

Author: Li, Hai; Wang, Fengjie; Han, Zongxi; Gao, Qi; Li, Huixin; Shao, Yuhao; Sun, Nana; Liu, Shengwang
Title: Genome-Wide Gene Expression Analysis Identifies the Proto-oncogene Tyrosine-Protein Kinase Src as a Crucial Virulence Determinant of Infectious Laryngotracheitis Virus in Chicken Cells
  • Document date: 2015_12_17
  • ID: qwrdr92h_11
    Snippet: Quantitation of virus. LMH cells were infected with LJS09 at a multiplicity of infection (MOI) of 0.1. The indicated MOI was obtained according to the number of cells to be infected and the number of infectious particles, which were estimated on the basis of the numbers of PFU, which were calculated using the following formula: TCID 50 ϫ 0.59 Ϸ PFU [1 TCID 50 is equal to Ϫln(0.5) PFU and is approximately 0.59 PFU] (40). Levels of virus replica.....
    Document: Quantitation of virus. LMH cells were infected with LJS09 at a multiplicity of infection (MOI) of 0.1. The indicated MOI was obtained according to the number of cells to be infected and the number of infectious particles, which were estimated on the basis of the numbers of PFU, which were calculated using the following formula: TCID 50 ϫ 0.59 Ϸ PFU [1 TCID 50 is equal to Ϫln(0.5) PFU and is approximately 0.59 PFU] (40). Levels of virus replication were determined using TCID 50 assays (40) on LMH cells and ILTV-specific qPCR assays (38) as previously described.

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