Author: Ahat, Erpan; Xiang, Yi; Zhang, Xiaoyan; Bekier, Michael E.; Wang, Yanzhuang
Title: GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of a5ß1 integrin Document date: 2019_3_15
ID: rfs7m6or_24
Snippet: To determine how GRASP depletion-mediated Golgi destruction affects cell growth, we examined the growth rate of GRASP-depleted cells using an established crystal violet assay (Tang et al., 2010) . Cells were transfected with indicated control or GRASP siRNAs and seeded into 24-well plates at 24 h after transfection. After incubation for 24 h, the number of cells was assessed for 6 successive days. As shown in Figure 7A , the increase in the cell .....
Document: To determine how GRASP depletion-mediated Golgi destruction affects cell growth, we examined the growth rate of GRASP-depleted cells using an established crystal violet assay (Tang et al., 2010) . Cells were transfected with indicated control or GRASP siRNAs and seeded into 24-well plates at 24 h after transfection. After incubation for 24 h, the number of cells was assessed for 6 successive days. As shown in Figure 7A , the increase in the cell number was significantly higher in cells treated with GRASP siRNAs compared with those treated with control siRNA.
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