Author: Hu, Song; Jiang, Li-Bin; Zou, Xiao-Jing; Yi, Wei; Tian, De-Ying
Title: Hepatitis B virus upregulates host expression of a-1,2-mannosidases via the PPARa pathway Document date: 2016_11_21
ID: w5m23ikh_6
Snippet: Plasmids containing seven individual viral genes of HBV (i.e., Ptt22-HBx, Ptt22-HBs, Ptt22-preS2, Ptt22-preS1, Ptt22-HBc, Ptt22-HBe, and Ptt22-HBp) and the control plasmid (Ptt22-vector) were a kind gift from Dr. Quan Yuan and Tianying Zhang (National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, China). Transient transfections with plasmids were performed using Lipofectamine 2000 Reagent (Invitrogen,.....
Document: Plasmids containing seven individual viral genes of HBV (i.e., Ptt22-HBx, Ptt22-HBs, Ptt22-preS2, Ptt22-preS1, Ptt22-HBc, Ptt22-HBe, and Ptt22-HBp) and the control plasmid (Ptt22-vector) were a kind gift from Dr. Quan Yuan and Tianying Zhang (National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, China). Transient transfections with plasmids were performed using Lipofectamine 2000 Reagent (Invitrogen, United States) according to the manufacturer's protocol. HepG2 cell lines only express PPARα and PPARγ but not PPARβ [18] . Therefore, PPARα (MK886) and PPARγ (GW9662) inhibitors were used at a concentration of 10 μmol/L, unless otherwise noted. MK886 and GW9662 (Sigma-Aldrich) were added to the cells 6 h after transfection.
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