Author: Ahat, Erpan; Xiang, Yi; Zhang, Xiaoyan; Bekier, Michael E.; Wang, Yanzhuang
Title: GRASP depletion–mediated Golgi destruction decreases cell adhesion and migration via the reduction of a5ß1 integrin Document date: 2019_3_15
ID: rfs7m6or_38
Snippet: For expression of exogenous GRASP proteins, HeLa cells of ∼60% confluency were transfected with the indicated GRASP constructs (Tang et al., 2010; Xiang and Wang, 2010) . For a 10-cm plate, 10 µg of pEGFP-N1-GRASP65 (WT) construct or 15 µg of pEGFP-N1-GRASP55 (WT) construct was mixed with 30 µl of polyethylenimine (PEI) and 1 ml of serum-free medium for 15 min at room temperature and then added to the cells in 9 ml of DMEM containing 10% sup.....
Document: For expression of exogenous GRASP proteins, HeLa cells of ∼60% confluency were transfected with the indicated GRASP constructs (Tang et al., 2010; Xiang and Wang, 2010) . For a 10-cm plate, 10 µg of pEGFP-N1-GRASP65 (WT) construct or 15 µg of pEGFP-N1-GRASP55 (WT) construct was mixed with 30 µl of polyethylenimine (PEI) and 1 ml of serum-free medium for 15 min at room temperature and then added to the cells in 9 ml of DMEM containing 10% super calf serum. For the control treatment, 10 µg of pEGFP-N1 construct was mixed with 30 µl of PEI and 1 ml of serum-free medium for 15 min and then added to the cells in 9 ml of DMEM containing 10% super calf serum.
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