Clinical Decision Support Tool and Rapid Point-of-Care Platform for Determining Disease Severity in Patients with COVID-19

Michael P McRae; Glennon W Simmons; Nicolaos J Christodoulides; Zhibing Lu; Stella K Kang; David Fenyo; Timothy Alcorn; Isaac P Dapkins; Iman Sharif; Deniz Vurmaz; Sayli S Modak; Kritika Srinivasan; Shruti Warhadpande; Ravi Shrivastav; John T McDevitt

is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04. 16.20068411 doi: medRxiv preprint in inset images on Figure 3 . Here, a single antigen standard at high concentration (1000ng/mL) was processed against a cartridge configured for multiplexed detection. As expected, monoclonal antibodies are highly specific for their target antigen, where high doses of each single antigen generated minimal cross-reactivity on non-target sensors. Although this work represents a subset of the full COVID-19 panel, the cartridge facilitates multiplexing of up to 20 different biomarkers and can be easily expanded to accommodate the panel and test validity controls. We anticipate that one or more of the selected six biomarkers may be dropped as additional COVID-19 clinical data are used to optimize the final COVID-19 Severity Score due to redundancy of patient discrimination information afforded by these biomarkers.