Author: Goodman, Laura B.; Anderson, Renee R.; Slater, Marcia; Ortenberg, Elen; Renshaw, Randall W.; Chilson, Brittany D.; Laverack, Melissa A.; Beeby, John S.; Dubovi, Edward J.; Glaser, Amy L.
Title: High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR Document date: 2016_11_28
ID: rd1bxkbu_1
Snippet: With the demand for rapid and comprehensive detection of multiple agents in clinical diagnostics for humans and animals, single-organism molecular diagnostic methods for pathogen detection are burdensome unless used for large numbers of samples being tested for a single disease. In the veterinary context, high-throughput diagnostic methodologies are particularly important due to the additional need for covering pathogens from a wide variety of sp.....
Document: With the demand for rapid and comprehensive detection of multiple agents in clinical diagnostics for humans and animals, single-organism molecular diagnostic methods for pathogen detection are burdensome unless used for large numbers of samples being tested for a single disease. In the veterinary context, high-throughput diagnostic methodologies are particularly important due to the additional need for covering pathogens from a wide variety of species. OneHealth approaches for management of food-borne disease and emerging pathogen surveillance are examples of testing needs that include a number of bacteria, viruses (DNA or RNA based), parasites, and fungi. The challenge to combining tests for multiple analytes together (multiplexing) in order to improve testing efficiency is a possible loss of sensitivity and a large burden for optimization and validation of assays.
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