Author: Goodman, Laura B.; Anderson, Renee R.; Slater, Marcia; Ortenberg, Elen; Renshaw, Randall W.; Chilson, Brittany D.; Laverack, Melissa A.; Beeby, John S.; Dubovi, Edward J.; Glaser, Amy L.
Title: High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR Document date: 2016_11_28
ID: rd1bxkbu_3
Snippet: We describe here a one-step preamp-based procedure for detecting both types of pathogens in respiratory secretions and a variety of other sample types that can be performed entirely in a standard workday. Upon completion of the one-step preamp, samples are transferred to a 384well plate, which is the format accepted by the automated liquid handling system that comes with this nanoscale PCR platform. The system draws the master mix and sample acro.....
Document: We describe here a one-step preamp-based procedure for detecting both types of pathogens in respiratory secretions and a variety of other sample types that can be performed entirely in a standard workday. Upon completion of the one-step preamp, samples are transferred to a 384well plate, which is the format accepted by the automated liquid handling system that comes with this nanoscale PCR platform. The system draws the master mix and sample across the surface of up to 4 plates (192 samples and controls) at a time. Following this loading process, we describe how samples are amplified and analyzed using a macro spreadsheet that summarizes the average of 3 technical replicates for each sample/target combination in a table that fits on one printed page.
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