Author: Bian, Qian; Lu, Jing; Zhang, Li; Chi, Ying; Li, Yan; Guo, Hongxiong
Title: Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway Document date: 2017_8_28
ID: s6kpewt6_6
Snippet: Cell line culture and transient transfection. Non-small cell lung cancer cell lines, NCI-H1299 and NCI-H292 (Cell Bank; Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences; Shanghai China), were separately grown as a monolayer in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen; Thermo Fischer Scientific, Inc.) supplemented with 10% fetal bovine serum (Invitrogen Thermo Fischer Scientific, Inc.) at 37ËšC and in a 5% CO 2.....
Document: Cell line culture and transient transfection. Non-small cell lung cancer cell lines, NCI-H1299 and NCI-H292 (Cell Bank; Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences; Shanghai China), were separately grown as a monolayer in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen; Thermo Fischer Scientific, Inc.) supplemented with 10% fetal bovine serum (Invitrogen Thermo Fischer Scientific, Inc.) at 37ËšC and in a 5% CO 2 incubator. The two cell lines were used for different purposes, NCI-H1299 was used to observe the localization of the NS1 protein in the cell whereas NCI-H292 was used to confirm the extent of apoptosis induced by the NS1 protein.
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