Selected article for: "bovine serum and ml penicillin"

Author: Wang, Huihui; Song, Hyo; Pham, Anha V.; Cooper, Laurence J.; Schulze, Janika J.; Olek, Sven; Tran, Dat Q.
Title: Human LAP(+)GARP(+)FOXP3(+) regulatory T cells attenuate xenogeneic graft versus host disease
  • Document date: 2019_4_12
  • ID: rudsvyqd_7
    Snippet: Anti-CD4, anti-biotin, anti-CD25 and anti-CD14 magnetic microbeads were from Miltenyi Biotec. All cells were cultured in complete media consisting of RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100 U/mL), streptomycin (100 μg/mL), 1×GlutaMAX (Gibco) and 1% MEM non-essential amino acids, 1% sodium pyruvate (Hyclone)......
    Document: Anti-CD4, anti-biotin, anti-CD25 and anti-CD14 magnetic microbeads were from Miltenyi Biotec. All cells were cultured in complete media consisting of RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100 U/mL), streptomycin (100 μg/mL), 1×GlutaMAX (Gibco) and 1% MEM non-essential amino acids, 1% sodium pyruvate (Hyclone).

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