Title: Hepatitis B surface antigen assembles in a post-ER, pre-Golgi compartment Document date: 1992_9_2
ID: qasgn7s9_16
Snippet: Double immunofluorescence labeling to study the distribution of rab2 and HBsAg in SV24 cells was performed essentially as described by Chavrier et al. (1990) . Free cytoplasmic rab2 was removed from the cells by incubating them for 4 min with 0.1% (wt/vol) saponin in 80 mM Pipes, pH 6.8, containing 5 mM EGTA and 1 mM MgC12. After fixation with 3% paraformaldebyde as described above, the cells were incubated with 0.1% (wt/vol) saponin in PBS for 5.....
Document: Double immunofluorescence labeling to study the distribution of rab2 and HBsAg in SV24 cells was performed essentially as described by Chavrier et al. (1990) . Free cytoplasmic rab2 was removed from the cells by incubating them for 4 min with 0.1% (wt/vol) saponin in 80 mM Pipes, pH 6.8, containing 5 mM EGTA and 1 mM MgC12. After fixation with 3% paraformaldebyde as described above, the cells were incubated with 0.1% (wt/vol) saponin in PBS for 5 min and quenched with 50 mM NH4C1 in PBS/saponin. The primary antibodies and the fluorescent labeled secondary antibodies were diluted in PBS/saponin and incubated as described by Chavrier et al. (1990) .
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