Author: Girardi, Erika; Chane-Woon-Ming, Béatrice; Messmer, Mélanie; Kaukinen, Pasi; Pfeffer, Sébastien
Title: Identification of RNase L-Dependent, 3'-End-Modified, Viral Small RNAs in Sindbis Virus-Infected Mammalian Cells Document date: 2013_11_19
ID: v6uc0ijw_25
Snippet: Recently, it has also been reported for several viruses that their genomic RNA can be 2=-O-methylated at specific positions (36, 37) . We hypothesized that the modification on the small RNA could be derived from a modification at the level of the genomic RNA, which is reasonable given the fact that SvsRNA-1 precursor RNAs also appear to be modified (see Fig. S7 in the supplemental material). We thus looked for modifications in the genomic region .....
Document: Recently, it has also been reported for several viruses that their genomic RNA can be 2=-O-methylated at specific positions (36, 37) . We hypothesized that the modification on the small RNA could be derived from a modification at the level of the genomic RNA, which is reasonable given the fact that SvsRNA-1 precursor RNAs also appear to be modified (see Fig. S7 in the supplemental material). We thus looked for modifications in the genomic region spanning SvsRNA-1 using the RTL-P (reverse transcription at low deoxyribonucleoside triphosphate concentrations followed by polymerase chain reaction) technique (38) : the tendency of the reverse transcriptase to pause immediately before a 2=-Omethylated nucleotide is exploited to establish the efficiency of retrotranscription by semiquantitative PCR, using differential deoxynucleoside triphosphate (dNTP) concentrations.
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