Author: He, Cheng; Kong, Ling; Zhou, Lijuan; Xia, Jing; Wei, Haixia; Liu, Min; Peng, Hongjuan
Title: Host Cell Vimentin Restrains Toxoplasma gondii Invasion and Phosphorylation of Vimentin is Partially Regulated by Interaction with TgROP18 Document date: 2017_9_5
ID: z9mg639h_53
Snippet: HBMEC and HBMEC ΔVim cells, or HFF cells pretreated with siRNA, were infected with T. gondii RH/GFP tachyzoites for 30 min, the non-invaded parasites were thoroughly washed off with PBS three times, and cells grown in DMEM complete medium for 22h. Subsequently, cell monolayers were fixed and examined to determine the number of vacuoles containing one, two, four, or eight parasites under a fluorescence microscope (100×). Means ± SD combined fro.....
Document: HBMEC and HBMEC ΔVim cells, or HFF cells pretreated with siRNA, were infected with T. gondii RH/GFP tachyzoites for 30 min, the non-invaded parasites were thoroughly washed off with PBS three times, and cells grown in DMEM complete medium for 22h. Subsequently, cell monolayers were fixed and examined to determine the number of vacuoles containing one, two, four, or eight parasites under a fluorescence microscope (100×). Means ± SD combined from three independent experiments, each performed in triplicate, were analyzed by two-way ANOVA [56] .
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