Title: Hepatitis B surface antigen assembles in a post-ER, pre-Golgi compartment Document date: 1992_9_2
ID: qasgn7s9_8
Snippet: Immunofluorescence and pulse-chase studies were performed in a mouse L cell line stably expressing HBsAg and no other viral proteins to localize the steps in the formation of these disulphide bonds. We show here that I-IBsAg is transported to a compartment which is intermediate between the ER and the Golgi and which excludes the major resident soluble ER proteins such as PDI but overlaps with rab2, a marker of an intermediate compartment. We also.....
Document: Immunofluorescence and pulse-chase studies were performed in a mouse L cell line stably expressing HBsAg and no other viral proteins to localize the steps in the formation of these disulphide bonds. We show here that I-IBsAg is transported to a compartment which is intermediate between the ER and the Golgi and which excludes the major resident soluble ER proteins such as PDI but overlaps with rab2, a marker of an intermediate compartment. We also show that the formation of the dimer crosslinks is completed in the ER while the formation of oligomer crosslinks occurs after exit from the ER and coincides with the budding of particles into the lumen of this intermediate compartment. We also demonstrate directly that the two classes of disulphide crosslinks have different energies since incubation of isolated, highly crosslinked particles with PDI causes the conversion of oligomers to dimers.
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