Selected article for: "anti rabbit and supernatant protein"
Author: Hu, Song; Jiang, Li-Bin; Zou, Xiao-Jing; Yi, Wei; Tian, De-Ying
Title: Hepatitis B virus upregulates host expression of a-1,2-mannosidases via the PPARa pathway
  • Document date: 2016_11_21
    • ID: w5m23ikh_10
    Snippet: Cells were lysed 72 h after transfection. Cell and tissue samples were lysed in RIRA (Sigma-Aldrich) buffer with PMSF (Sigma-Aldrich). Lysates were centrifuged for 10 min at 12000 × g, and protein in the supernatant was quantified using the BCA method (Pierce). SDS-PAGE and western blot analysis were performed according to standard procedures. Mouse anti-HBs, anti-HBx, anti-HBp, and anti-HBc antibodies were kindly provided by Dr. Quan Yuan from .....
    Document: Cells were lysed 72 h after transfection. Cell and tissue samples were lysed in RIRA (Sigma-Aldrich) buffer with PMSF (Sigma-Aldrich). Lysates were centrifuged for 10 min at 12000 × g, and protein in the supernatant was quantified using the BCA method (Pierce). SDS-PAGE and western blot analysis were performed according to standard procedures. Mouse anti-HBs, anti-HBx, anti-HBp, and anti-HBc antibodies were kindly provided by Dr. Quan Yuan from Xiamen University. Goat anti-MAN1A1 (Santa Cruz; 1:500 dilution), rabbit anti-MAN1A2 (Proteintech; 1:200 dilution), rabbit anti-MAN1B1 (GeneTex; 1:500 dilution), mouse anti-MAN1C1 (Abcam; 1:500 dilution) antibodies were used as the primary antibodies. Mouse anti-β-actin (Proteintech; 1:1000 dilution) was used as a reference for protein quantification. Goat anti-mouse IgG (Proteintech; 1:10000 dilution), goat anti-rabbit IgG (Proteintech; 1:10000 dilution), and donkey antigoat IgG (Santa Cruz; 1:10000 dilution) were used as the secondary antibodies followed by enhanced chemiluminescence (ECL; ThermoFisher Scientific) detection.

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