Selected article for: "room temperature and viral titer"
Author: Than, Thoa Thi; Jo, Eunji; Todt, Daniel; Nguyen, Phuong Hong; Steinmann, Jochen; Steinmann, Eike; Windisch, Marc P
Title: High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides
  • Document date: 2019_4_1
    • ID: y9ezh49z_13
    Snippet: As HBV particles in the infected host are circulating in the presence of serum, the effect of healthy human serum (50%) on HBV stability was analyzed by incubation of viral particles at room temperature for 7 days and comparing them to HBV incubated side by side in 10% FBS. The addition of human serum did not reduce HBV infectivity over time and was comparable to findings of incubation with FBS ( Figure 1C ). Next, the impact of changes in the pH.....
    Document: As HBV particles in the infected host are circulating in the presence of serum, the effect of healthy human serum (50%) on HBV stability was analyzed by incubation of viral particles at room temperature for 7 days and comparing them to HBV incubated side by side in 10% FBS. The addition of human serum did not reduce HBV infectivity over time and was comparable to findings of incubation with FBS ( Figure 1C ). Next, the impact of changes in the pH on viral stability was tested by incubating HBV at pH 3-8 for 10 minutes at 37°C. Afterward, the viral titer was determined by a limiting dilution assay, which demonstrated that HBV infectivity was not affected at pH 3-8 ( Figure 1D ).

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