Author: Bian, Qian; Lu, Jing; Zhang, Li; Chi, Ying; Li, Yan; Guo, Hongxiong
Title: Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway Document date: 2017_8_28
ID: s6kpewt6_7
Snippet: NCI-H1299 and NCI-H292 were transfected with pXJ40-HA-NS1 or control plasmids (pXJ40-HA-vector; Invi trogen; Thermo Fischer Scientific, Inc.) using Lipofectamine 2000 reagent according to the manufacturer's instructions (Invitrogen; Thermo Fisher Scientific, Inc). After 4 h, Lipofectamine 2000-DNA complexes were removed, and the cell culture DMEM medium was replaced with fresh DMEM with or without 0.025 nM staurosporine (STS; Sigma-Aldrich; Merk .....
Document: NCI-H1299 and NCI-H292 were transfected with pXJ40-HA-NS1 or control plasmids (pXJ40-HA-vector; Invi trogen; Thermo Fischer Scientific, Inc.) using Lipofectamine 2000 reagent according to the manufacturer's instructions (Invitrogen; Thermo Fisher Scientific, Inc). After 4 h, Lipofectamine 2000-DNA complexes were removed, and the cell culture DMEM medium was replaced with fresh DMEM with or without 0.025 nM staurosporine (STS; Sigma-Aldrich; Merk KGaA, Darmstadt, Germany) at 37ËšC for 24 h. Cells were collected after 24 h, washed with phosphate-buffered saline (PBS) and trypsinized with 0.125% trypsin/EDTA solution.
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