Author: Bian, Qian; Lu, Jing; Zhang, Li; Chi, Ying; Li, Yan; Guo, Hongxiong
Title: Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway Document date: 2017_8_28
ID: s6kpewt6_10
Snippet: Flow cytometric analysis. In order to determine the apoptotic rate, ~1x10 6 NCI-H292 cells/ml were stained with fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (556547, Annexin V-FITC apoptosis detection kit, BD Pharmingen; BD Biosciences) in a volume of 100 µl on ice for 30 min in the dark. The cells were washed 3 times with PBS. Finally, 400 µl binding buffer was added to the cells. Additionally, the mixture was an.....
Document: Flow cytometric analysis. In order to determine the apoptotic rate, ~1x10 6 NCI-H292 cells/ml were stained with fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (556547, Annexin V-FITC apoptosis detection kit, BD Pharmingen; BD Biosciences) in a volume of 100 µl on ice for 30 min in the dark. The cells were washed 3 times with PBS. Finally, 400 µl binding buffer was added to the cells. Additionally, the mixture was analyzed with a BD FACSCalibur flow cytometer and the percentage of apoptosis of 10,000 cells was determined. The data were analyzed using BD CellQuest™ Pro software (version 5.2.1; BD Biosciences) and the percentage cells with apoptosis per group were calculated.
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