Author: Bian, Qian; Lu, Jing; Zhang, Li; Chi, Ying; Li, Yan; Guo, Hongxiong
Title: Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway Document date: 2017_8_28
ID: s6kpewt6_19
Snippet: To further confirm cell apoptosis, which is associated with NS1 protein expression, the NCI-H292 cells expressing NS1 protein were stained with Annexin V-FITC and PI, and detected using flow cytometry (Fig. 3) . Compared with the empty vector-transfected cells (9.19% Annexin V + /PI -, indicating early apoptosis, and 4.49% Annexin V + /PI + , indicating late apoptosis), the NS1-transfected cells were 22.96% Annexin V + /PIand 2.62% Annexin V + /P.....
Document: To further confirm cell apoptosis, which is associated with NS1 protein expression, the NCI-H292 cells expressing NS1 protein were stained with Annexin V-FITC and PI, and detected using flow cytometry (Fig. 3) . Compared with the empty vector-transfected cells (9.19% Annexin V + /PI -, indicating early apoptosis, and 4.49% Annexin V + /PI + , indicating late apoptosis), the NS1-transfected cells were 22.96% Annexin V + /PIand 2.62% Annexin V + /PI + . Following treatment with STS, NS1-transfected cells were 43.35% Annexin V + /PIand 2.98% Annexin V + /PI + , whereas empty vector-transfected cells were 33.47% Annexin V + /PIand 8.16% Annexin V + /PI + . Compared with the empty vector-transfected group, the number of apoptotic cells in the NS1-plasmid transfected group was significantly increased (P<0.05). Following combinational treatment of NS1 and STS, the number of apoptotic cells was significantly increased compared with the NS1 group (P<0.05). These results indicate that NS1 is associated with the apoptosis of NCI-H292 cells, and that the effect of apoptosis induced by NS1 protein may be enhanced by STS.
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