Author: Stenglein, Mark D.; Sanders, Chris; Kistler, Amy L.; Ruby, J. Graham; Franco, Jessica Y.; Reavill, Drury R.; Dunker, Freeland; DeRisi, Joseph L.
Title: Identification, Characterization, and In Vitro Culture of Highly Divergent Arenaviruses from Boa Constrictors and Annulated Tree Boas: Candidate Etiological Agents for Snake Inclusion Body Disease Document date: 2012_8_14
ID: vkhg20he_39
Snippet: Tissue culture. VH-2 and IGH-2 cells were obtained from the ATCC and were grown at 30°C in Eagle's minimum essential medium with Hanks' balanced salt solution (University of California San Francisco [UCSF] cell culture facility) supplemented with 10% fetal bovine serum, 50 units/ml penicillin, 50 g/ml streptomycin, and 25 mM HEPES (pH 7.4) (cMEM). Vero cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine seru.....
Document: Tissue culture. VH-2 and IGH-2 cells were obtained from the ATCC and were grown at 30°C in Eagle's minimum essential medium with Hanks' balanced salt solution (University of California San Francisco [UCSF] cell culture facility) supplemented with 10% fetal bovine serum, 50 units/ml penicillin, 50 g/ml streptomycin, and 25 mM HEPES (pH 7.4) (cMEM). Vero cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 50 units/ml penicillin, 50 g/ml streptomycin at 37°C and 5% CO 2 .
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