Author: Lippens, Carla; Duraes, Fernanda V.; Dubrot, Juan; Brighouse, Dale; Lacroix, Mathilde; Irla, Magali; Aubry-Lachainaye, Jean-Pierre; Reith, Walter; Mandl, Judith N.; Hugues, Stéphanie
Title: IDO-orchestrated crosstalk between pDCs and Tregs inhibits autoimmunity Document date: 2016_12_23
ID: sl8148ap_21
Snippet: pDCs were generated from BM of WT, pIII + IV−/− and IDO−/− mice as previously described [23]. Briefly, BM cells were recovered from tibia and femurs of mice and cultured, after red cell lysis, for 7 days in complete RPMI medium (10% heat-inactivated fetal bovine serum, 50 mM 2-βMercaptoethanol, 100 mM sodium Pyruvate and 100 μM of Penicillin/Streptomycin) supplemented with 100 ng/mL of murine Flt3L (PeproTech)......
Document: pDCs were generated from BM of WT, pIII + IV−/− and IDO−/− mice as previously described [23]. Briefly, BM cells were recovered from tibia and femurs of mice and cultured, after red cell lysis, for 7 days in complete RPMI medium (10% heat-inactivated fetal bovine serum, 50 mM 2-βMercaptoethanol, 100 mM sodium Pyruvate and 100 μM of Penicillin/Streptomycin) supplemented with 100 ng/mL of murine Flt3L (PeproTech).
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