IgY antibodies for the immunoprophylaxis and therapy of respiratory infections

Abbas, Aymn Talat; El-Kafrawy, Sherif Aly; Sohrab, Sayed Sartaj; Azhar, Esam Ibraheem Ahmed

The promise of IgY antibodies underscores the need for continuing research to improve different aspects of production, including chicken immunization protocols and adjuvant use, IgY extraction techniques, and methods to increase antibody yield. Development of monoclonal IgY antibodies will combine the advantages of both mAbs and avian IgY. Chicken mAbs are antibodies derived from a single B-cell lineage that has undergone repetitive rounds of somatic hypermutation and clonal selection and are usually of the IgY isotype recognizing a single unique epitope. 124 Thus, chicken mAbs tend to be more specific and of higher affinity compared to polyclonal antibodies counterparts. 125 Genetically engineered singlechain fragment variable IgY (IgY-scFv) has been successfully generated based on phage display technologies by different research groups. 26 Spillner et al 126