Author: Goodman, Laura B.; Anderson, Renee R.; Slater, Marcia; Ortenberg, Elen; Renshaw, Randall W.; Chilson, Brittany D.; Laverack, Melissa A.; Beeby, John S.; Dubovi, Edward J.; Glaser, Amy L.
Title: High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR Document date: 2016_11_28
ID: rd1bxkbu_20
Snippet: The analytical limit of detection (LOD) for each target was calculated based on the overall mean of the Ct values at the 95% detection level plus 2 standard deviations in a pool of controls for all targets. The highest dilution where at least 95% of the replicates were positive for the representative assays was 50 copies; detection of 5 copies was successful in 50% of replicates. Neither assay was detected at below one copy. The LODs for the RNA .....
Document: The analytical limit of detection (LOD) for each target was calculated based on the overall mean of the Ct values at the 95% detection level plus 2 standard deviations in a pool of controls for all targets. The highest dilution where at least 95% of the replicates were positive for the representative assays was 50 copies; detection of 5 copies was successful in 50% of replicates. Neither assay was detected at below one copy. The LODs for the RNA and DNA assay were calculated at Ct values of 21.92 and 20.05. These values were considered to be the cutoffs for reporting values as positive vs. suspect (potentially not repeatable).
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