Selected article for: "cell culture and real time"
Author: Hu, Song; Jiang, Li-Bin; Zou, Xiao-Jing; Yi, Wei; Tian, De-Ying
Title: Hepatitis B virus upregulates host expression of a-1,2-mannosidases via the PPARa pathway
  • Document date: 2016_11_21
    • ID: w5m23ikh_4
    Snippet: Human hepatocellular carcinoma cells (HepG2, HepG2.2.15, AD38, and N10) were cultured in DMEM at 37 ℃ in a 5% CO2 incubator. The medium was supplemented with 10% FBS, 100 IU/mL penicillin, and 100 IU/mL streptomycin. Cells were changed into fresh medium every third day, and split by trypsinization at a confluence of about 90% [15] . AD38 cells, which are a variant of HepG2 cells, express the HBV genome under the control of a tetracycline (Tet)-.....
    Document: Human hepatocellular carcinoma cells (HepG2, HepG2.2.15, AD38, and N10) were cultured in DMEM at 37 ℃ in a 5% CO2 incubator. The medium was supplemented with 10% FBS, 100 IU/mL penicillin, and 100 IU/mL streptomycin. Cells were changed into fresh medium every third day, and split by trypsinization at a confluence of about 90% [15] . AD38 cells, which are a variant of HepG2 cells, express the HBV genome under the control of a tetracycline (Tet)-off promoter. Therefore, the AD38 cell culture medium also contained tetracycline (1 μg/mL) when not requiring the expression of HBV genes [16] . HepG2.2.15 and N10 cells are secretory HBV cell lines derived from G2 [17] . Viral antigens (HBsAg and HBeAg) in the culture medium were measured using the chemiluminescence method with commercial assay kits (Wantai, Beijing, China). HBV DNA quantification assays were performed using a commercial real-time PCR kit (Kehua, Shanghai, China).

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