High Environmental Stability of Hepatitis B Virus and Inactivation Requirements for Chemical Biocides

Than, Thoa Thi; Jo, Eunji; Todt, Daniel; Nguyen, Phuong Hong; Steinmann, Jochen; Steinmann, Eike; Windisch, Marc P

To investigate the effect of different temperatures on HBV infectivity, viral suspensions were exposed to different temperatures at indicated time points in 0.2-mL polymerase chain reaction tubes (Eppendorf, Germany), using Takara Thermal Cycler B R I E F R E P O R T © The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/ by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact [e-mail] DOI: 10.1093/infdis/jiy620 Dice Touch (Takara, Australia). After heat treatment, samples were immediately kept on ice before infection of the cells. To examine the impact of different pH values, 6 solutions with different pH values were prepared as described elsewhere [9] . The virus was incubated with different solutions at 37°C for 10 minutes at a volume ratio of 1:4. The viral suspension was then immediately serially diluted in Dulbecco's modified Eagle medium and then titrated in 384-well microplates.