Author: Bian, Qian; Lu, Jing; Zhang, Li; Chi, Ying; Li, Yan; Guo, Hongxiong
Title: Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway Document date: 2017_8_28
ID: s6kpewt6_9
Snippet: Cell viability assay. MTT cell viability assays were performed according to the manufacturer's instructions (Sigma-Aldrich; Merck KGaA). Briefly, 20 µl 5 mg/ml MTT was added to the culture medium. Following incubation at 37˚C for 3 h, 100 µl acidic isopropanol (0.1 nM HCl in acidic isopropanol) was added to the NCI-H292 cells and then the absorbance of each sample was measured at 570 nm with an automated plate reader (SpectraMax Paradigm; Mole.....
Document: Cell viability assay. MTT cell viability assays were performed according to the manufacturer's instructions (Sigma-Aldrich; Merck KGaA). Briefly, 20 µl 5 mg/ml MTT was added to the culture medium. Following incubation at 37˚C for 3 h, 100 µl acidic isopropanol (0.1 nM HCl in acidic isopropanol) was added to the NCI-H292 cells and then the absorbance of each sample was measured at 570 nm with an automated plate reader (SpectraMax Paradigm; Molecular Devices, LLC, Sunnyvale, CA, USA) compared to the same number of control cells.
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