Selected article for: "internal control and Trizol reagent"
Author: He, Cheng; Kong, Ling; Zhou, Lijuan; Xia, Jing; Wei, Haixia; Liu, Min; Peng, Hongjuan
Title: Host Cell Vimentin Restrains Toxoplasma gondii Invasion and Phosphorylation of Vimentin is Partially Regulated by Interaction with TgROP18
  • Document date: 2017_9_5
    • ID: z9mg639h_49
    Snippet: Levels of vimentin mRNA were compared by qRT-PCR to determine the efficiency of RNA interference in HFF cells and transcription levels of vimentin in different mouse tissues. Total RNA samples were extracted from tissues or HFF cells using Trizol ® Reagent (Ambion TM ) and cDNAs were synthesized using the gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech, China). qRT-PCR was performed using Top Green qPCR SuperMix (TransGen Biotech, Chi.....
    Document: Levels of vimentin mRNA were compared by qRT-PCR to determine the efficiency of RNA interference in HFF cells and transcription levels of vimentin in different mouse tissues. Total RNA samples were extracted from tissues or HFF cells using Trizol ® Reagent (Ambion TM ) and cDNAs were synthesized using the gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech, China). qRT-PCR was performed using Top Green qPCR SuperMix (TransGen Biotech, China) according to the manufacturer's instructions. The primers used are listed in Table S2 . Beta-actin was used as an internal control for normalization.

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