Author: He, Cheng; Kong, Ling; Zhou, Lijuan; Xia, Jing; Wei, Haixia; Liu, Min; Peng, Hongjuan
Title: Host Cell Vimentin Restrains Toxoplasma gondii Invasion and Phosphorylation of Vimentin is Partially Regulated by Interaction with TgROP18 Document date: 2017_9_5
ID: z9mg639h_30
Snippet: All T. gondii strains used in this research were derived from the type I RH line and tachyzoites were maintained by growth in human foreskin fibroblasts (HFF). The HFF cell line was obtained from American Type Culture Collection (ATCC), and grown in Dulbecco's Modified Eagles Medium (DMEM, Thermo Fisher Scientific) supplemented with 1% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific). Transgenic parasites were obtained by electroporatio.....
Document: All T. gondii strains used in this research were derived from the type I RH line and tachyzoites were maintained by growth in human foreskin fibroblasts (HFF). The HFF cell line was obtained from American Type Culture Collection (ATCC), and grown in Dulbecco's Modified Eagles Medium (DMEM, Thermo Fisher Scientific) supplemented with 1% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific). Transgenic parasites were obtained by electroporation of recombinant plasmids into wild type RH tachyzoites and selection with 3μM pyrimethamine (Sigma). Freshly egressed parasites purified with 3μm nucleopore filters were used in all experiments.
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