Author: He, Cheng; Kong, Ling; Zhou, Lijuan; Xia, Jing; Wei, Haixia; Liu, Min; Peng, Hongjuan
Title: Host Cell Vimentin Restrains Toxoplasma gondii Invasion and Phosphorylation of Vimentin is Partially Regulated by Interaction with TgROP18 Document date: 2017_9_5
ID: z9mg639h_57
Snippet: Bacterially derived, purified GST-ROP18 (200ng) was incubated with vimentin (3μg) in reaction buffer (1mM unlabeled ATP, 25mM Tris/HCl (pH 7.5), 15mM MgCl 2 ) for 30min at 30°C. Then, samples were immediately boiled in sample buffer for 5 min for western blot analysis. Equal purified GST was incubated with vimentin and detected as control according to above description......
Document: Bacterially derived, purified GST-ROP18 (200ng) was incubated with vimentin (3μg) in reaction buffer (1mM unlabeled ATP, 25mM Tris/HCl (pH 7.5), 15mM MgCl 2 ) for 30min at 30°C. Then, samples were immediately boiled in sample buffer for 5 min for western blot analysis. Equal purified GST was incubated with vimentin and detected as control according to above description.
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