Selected article for: "background fluorescence and cycle threshold"

Author: Goodman, Laura B.; Anderson, Renee R.; Slater, Marcia; Ortenberg, Elen; Renshaw, Randall W.; Chilson, Brittany D.; Laverack, Melissa A.; Beeby, John S.; Dubovi, Edward J.; Glaser, Amy L.
Title: High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
  • Document date: 2016_11_28
  • ID: rd1bxkbu_19
    Snippet: Results are shown in Figures 1 and 2 for a representative RNA assay (influenza matrix) and DNA assay (EHV-1) with a combination of positive controls and clinical samples submitted for routine diagnostic testing. The fluorescence signals emitted throughout this typical reaction are shown in Figure 1 , which plots raw fluorescence values per cycle. All relative cycle threshold (Ct) values were automatically generated by the amplification machine so.....
    Document: Results are shown in Figures 1 and 2 for a representative RNA assay (influenza matrix) and DNA assay (EHV-1) with a combination of positive controls and clinical samples submitted for routine diagnostic testing. The fluorescence signals emitted throughout this typical reaction are shown in Figure 1 , which plots raw fluorescence values per cycle. All relative cycle threshold (Ct) values were automatically generated by the amplification machine software. Background fluorescence was used as a separate metric for assessment of loading quality rather than incorporating it into the fluorescence readings prior to calculating Ct values.

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