Author: Goodman, Laura B.; Anderson, Renee R.; Slater, Marcia; Ortenberg, Elen; Renshaw, Randall W.; Chilson, Brittany D.; Laverack, Melissa A.; Beeby, John S.; Dubovi, Edward J.; Glaser, Amy L.
Title: High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR Document date: 2016_11_28
ID: rd1bxkbu_2
Snippet: Nanoliter scale real-time polymerase chain reaction (PCR) is an alternative to the practice of multiplexing that allows many separate reactions to run simultaneously on the same sample 1 . The OpenArray platform is an application of this principle 2 ; it combines microarray technology with real-time PCR. Based on the concept of spatial multiplexing, every sample is tested for a large number of targets in separate through-holes. This platform was .....
Document: Nanoliter scale real-time polymerase chain reaction (PCR) is an alternative to the practice of multiplexing that allows many separate reactions to run simultaneously on the same sample 1 . The OpenArray platform is an application of this principle 2 ; it combines microarray technology with real-time PCR. Based on the concept of spatial multiplexing, every sample is tested for a large number of targets in separate through-holes. This platform was initially used with cyanine-based double-stranded DNA binding chemistry 2 and is now available for probe-based chemistry using dark quenching probes 3 . This platform has primarily been used for genetic profiling in humans 4 and animals 5 . It was recently adapted to detect blood-borne DNA and RNA pathogens by Grigorenko et al. 6 using a two-step reverse-transcription/pre-amplification (preamp) procedure.
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