Selected article for: "real time and RNA Extraction kit"
Author: Huh, Hee Jae; Ko, Jae-Hoon; Kim, Young-Eun; Park, Chang-Hun; Hong, Geehay; Choi, Rihwa; Yu, Shinae; Cho, Sun Young; Kang, Ji-Man; Lee, Myoung-Keun; Ki, Chang-Seok; Kang, Eun-Suk; Lee, Nam Yong; Kim, Jong-Won; Kim, Yae-Jean; Ha, Young Eun; Kang, Cheol-In; Chung, Doo Ryeon; Peck, Kyong Ran; Song, Jae-Hoon
Title: Importance of Specimen Type and Quality in Diagnosing Middle East Respiratory Syndrome
  • Document date: 2016_11_1
    • ID: s958dq2q_2
    Snippet: From June 1 to July 5, 2015, clinical specimens were screened by rRT-PCR testing targeting the upstream E region (upE) using PowerChek MERS real-time PCR kits (Kogene Biotech, Seoul, Korea) after viral RNA extraction using the QIAamp DSP Viral RNA Mini kit (Qiagen cat# 61904; Qiagen, Hilden, Germany) or the MagNA Pure 96 system (Roche Applied Science, Manheim, Germany) according to the manufacturer's instructions. Screenpositive specimens were co.....
    Document: From June 1 to July 5, 2015, clinical specimens were screened by rRT-PCR testing targeting the upstream E region (upE) using PowerChek MERS real-time PCR kits (Kogene Biotech, Seoul, Korea) after viral RNA extraction using the QIAamp DSP Viral RNA Mini kit (Qiagen cat# 61904; Qiagen, Hilden, Germany) or the MagNA Pure 96 system (Roche Applied Science, Manheim, Germany) according to the manufacturer's instructions. Screenpositive specimens were confirmed by the subsequent amplification of ORF1a. A positive test result was defined as a well-defined exponential fluorescence curve that crossed a threshold of ≤ 35 cycles for both upE and ORF1a. For inconclusive results, a specimen was considered "equivocal" if the upE result was positive, but the cycle threshold (Ct) value for ORF1a was between 35 and 40. Additionally, we interpreted the result as "indeterminate" if: 1) the upE result was positive, but the Ct value for ORF1a was undetected or 2) the Ct value for upE was between 35 and 40 [3, 9] .

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