Author: Ortega, Ariadna Flores; Martínez-Castañeda, José Simón; Bautista-Gómez, Linda G.; Muñoz, Raúl Fajardo; Hernández, Israel Quijano
Title: Identification of co-infection by rotavirus and parvovirus in dogs with gastroenteritis in Mexico Document date: 2017_6_24
ID: rhsne157_9
Snippet: In order to verify primer specificity, amplicons from positive controls were sequenced. Sequencing was accomplished through the following procedure: first, PCR products were purified from agarose gel using the Wizard ® SV Gel and PCR The sequences obtained were matched 100% with the Gen-Bank sequences (Fig. 2) , which indicates that primers used for PCR and RT-PCR in this study, are specific for genome amplification of both viruses. Therefore, t.....
Document: In order to verify primer specificity, amplicons from positive controls were sequenced. Sequencing was accomplished through the following procedure: first, PCR products were purified from agarose gel using the Wizard ® SV Gel and PCR The sequences obtained were matched 100% with the Gen-Bank sequences (Fig. 2) , which indicates that primers used for PCR and RT-PCR in this study, are specific for genome amplification of both viruses. Therefore, the analysis of all samples was accomplished using both PCR and RT-PCR protocols. Out of 50 dogs, 82% (45/50) were positive for at least one of the two viruses, specifically, 60% (27/45) were positive only for parvovirus, 8% (7/45) were positive only for rotavirus and 14% (11/45) were co-infected. Five samples, which were not positive for either parvovirus or rotavirus, were positive for canine distemper or Salmonella spp. (data not shown).
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