Author: Girardi, Erika; Chane-Woon-Ming, Béatrice; Messmer, Mélanie; Kaukinen, Pasi; Pfeffer, Sébastien
Title: Identification of RNase L-Dependent, 3'-End-Modified, Viral Small RNAs in Sindbis Virus-Infected Mammalian Cells Document date: 2013_11_19
ID: v6uc0ijw_20
Snippet: Next, we performed sodium periodate (NaIO 4 ) treatment followed by â¤-elimination to specifically examine the 3= end of SvsRNA-1. The presence of hydroxyl groups in positions 2= and (B and E) SINV genomic RNA was quantified by RT-qPCR, before and after RNase L or Xrn1 KD. The relative quantification is normalized to GAPDH levels and is represented on a logarithmic scale as a mean of three replicates. (C and F) Northern blot analysis on total RN.....
Document: Next, we performed sodium periodate (NaIO 4 ) treatment followed by â¤-elimination to specifically examine the 3= end of SvsRNA-1. The presence of hydroxyl groups in positions 2= and (B and E) SINV genomic RNA was quantified by RT-qPCR, before and after RNase L or Xrn1 KD. The relative quantification is normalized to GAPDH levels and is represented on a logarithmic scale as a mean of three replicates. (C and F) Northern blot analysis on total RNA from infected HEK293 cells before and after RNase L or Xrn1 KD. U6 was used as a normalizer.
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