Author: Girardi, Erika; Chane-Woon-Ming, Béatrice; Messmer, Mélanie; Kaukinen, Pasi; Pfeffer, Sébastien
Title: Identification of RNase L-Dependent, 3'-End-Modified, Viral Small RNAs in Sindbis Virus-Infected Mammalian Cells Document date: 2013_11_19
ID: v6uc0ijw_30
Snippet: After deep sequencing of these libraries, we mapped the total number of reads against both the human and SINV genome (Table 1). As expected, in both noninfected and infected cells, the NaIO 4 treatment drastically reduced the number of clonable miRNA sequences (see also Fig. S9A in the supplemental material). In contrast, modified small RNAs (such as tRNA fragments) were preferentially cloned over miRNAs upon NaIO 4 treatment (Fig. S9B) . Strikin.....
Document: After deep sequencing of these libraries, we mapped the total number of reads against both the human and SINV genome (Table 1). As expected, in both noninfected and infected cells, the NaIO 4 treatment drastically reduced the number of clonable miRNA sequences (see also Fig. S9A in the supplemental material). In contrast, modified small RNAs (such as tRNA fragments) were preferentially cloned over miRNAs upon NaIO 4 treatment (Fig. S9B) . Strikingly, almost 75% of the viral reads were kept after oxidation ( Table 1 ). The oxidation treatment did not dramatically change the genomic distribution of the viral reads compared to the nontreated sample (Fig. 5A) . However, deep sequencing revealed some new peaks (Fig. 5A, arrows) that we could also validate by â¤-elimination and Northern blot analysis (see SvsRNA-15 and -16 in Fig. S8) .
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