Author: Zeng, Zhengyang; Zhang, Runhong; Hong, Wei; Cheng, Yuting; Wang, Huijuan; Lang, Yange; Ji, Zhenglin; Wu, Yingliang; Li, Wenxin; Xie, Youli; Cao, Zhijian
Title: Histidine-rich Modification of a Scorpion-derived Peptide Improves Bioavailability and Inhibitory Activity against HSV-1 Document date: 2018_1_1
ID: zilqyfjl_19
Snippet: Six-well plates containing 80-90% confluent Vero cell monolayers were infected with 10-fold diluted series of HSV-1 aliquots. After incubation at 37 ℃ for 1 h, the inocula were removed. The cells were washed three times with PBS and replenished with a maintenance cover layer (MEM with 2% FBS and 0.75% carboxy-methylcellulose). After an incubation period of 72 h, the cells were stained with 1% crystal violet containing 10% methanol, and the plaq.....
Document: Six-well plates containing 80-90% confluent Vero cell monolayers were infected with 10-fold diluted series of HSV-1 aliquots. After incubation at 37 ℃ for 1 h, the inocula were removed. The cells were washed three times with PBS and replenished with a maintenance cover layer (MEM with 2% FBS and 0.75% carboxy-methylcellulose). After an incubation period of 72 h, the cells were stained with 1% crystal violet containing 10% methanol, and the plaque numbers were visualized. The viral titer in plaque-forming units (PFU) was then calculated according to the plaque number and dilution ratio. The details of the antiviral assays are described in the section below.
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