Author: Zeng, Zhengyang; Zhang, Runhong; Hong, Wei; Cheng, Yuting; Wang, Huijuan; Lang, Yange; Ji, Zhenglin; Wu, Yingliang; Li, Wenxin; Xie, Youli; Cao, Zhijian
Title: Histidine-rich Modification of a Scorpion-derived Peptide Improves Bioavailability and Inhibitory Activity against HSV-1 Document date: 2018_1_1
ID: zilqyfjl_65
Snippet: In this study, we screened the cDNA library from the scorpion venomous gland and identified six scorpion venom peptides, 12-14 amino acids in length, as antimicrobial candidates. The viral inactivation activities of these six scorpion venom peptides against HSV-1 were screened by PRA assay. Eval418, which contained 13 amino acid residues, cleared almost 100% of PFUs. The cytotoxicity and hemolysis activity of Eval418 were further tested, and the .....
Document: In this study, we screened the cDNA library from the scorpion venomous gland and identified six scorpion venom peptides, 12-14 amino acids in length, as antimicrobial candidates. The viral inactivation activities of these six scorpion venom peptides against HSV-1 were screened by PRA assay. Eval418, which contained 13 amino acid residues, cleared almost 100% of PFUs. The cytotoxicity and hemolysis activity of Eval418 were further tested, and the results showed that Eval418 is minimally toxic at a concentration of 10 μg/mL. Furthermore, Eval418 showed a significant dose-dependent inhibition when pre-incubated with HSV-1 or added during the HSV-1 viral attachment step with IC50 values of 2.48 and 3.70 μg/mL, respectively.
Search related documents:
Co phrase search for related documents- amino acid and cdna library: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- amino acid and cdna library screen: 1, 2
- amino acid and dose dependent inhibition: 1, 2, 3
- amino acid and inactivation activity: 1, 2
- amino acid and length amino acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- amino acid and pre incubate: 1
- amino acid and scorpion venom: 1, 2
- amino acid residue and length amino acid: 1
- antimicrobial candidate and cdna library: 1
- cdna library and dose dependent inhibition: 1
- cdna library and inactivation activity: 1
- cdna library and scorpion venom: 1
- cdna library and scorpion venom peptide: 1
- cdna library and venom peptide: 1, 2
- dose dependent inhibition and inactivation activity: 1, 2, 3
- dose dependent inhibition and scorpion venom: 1
- dose dependent inhibition and scorpion venom peptide: 1
- dose dependent inhibition and significant dose dependent inhibition: 1, 2
- dose dependent inhibition and venom peptide: 1
Co phrase search for related documents, hyperlinks ordered by date