Selected article for: "anti mouse and cell number"
Author: He, Cheng; Kong, Ling; Zhou, Lijuan; Xia, Jing; Wei, Haixia; Liu, Min; Peng, Hongjuan
Title: Host Cell Vimentin Restrains Toxoplasma gondii Invasion and Phosphorylation of Vimentin is Partially Regulated by Interaction with TgROP18
  • Document date: 2017_9_5
    • ID: z9mg639h_51
    Snippet: Invasion assays were performed based on differential staining of intracellular vs. extracellular parasites, as previously described [8] . T. gondii RH/GFP tachyzoites harvested by mechanical lysis were allowed to invade HBMEC and HBMEC ΔVim monolayers for 30 min at 37°C and then fixed with 4% paraformaldehyde. Extracellular parasites were detected by staining with mouse anti-SAG1antibody. Both intracellular and extracellular parasites expressin.....
    Document: Invasion assays were performed based on differential staining of intracellular vs. extracellular parasites, as previously described [8] . T. gondii RH/GFP tachyzoites harvested by mechanical lysis were allowed to invade HBMEC and HBMEC ΔVim monolayers for 30 min at 37°C and then fixed with 4% paraformaldehyde. Extracellular parasites were detected by staining with mouse anti-SAG1antibody. Both intracellular and extracellular parasites expressing GFP were observed by fluorescence microscopy. Slides were examined by epifluorescence microscopy and the total numbers of parasites (green), extracellular parasites (red), and host cell nuclei (blue) were counted from counts of ≥200 parasites. Values are expressed as the average number of parasite/host cell nuclei and represent means ± SD of three independent experiments.

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