Author: Kim, Jang Su; Lim, Chae Seung; Kim, Young Kee; Lee, Kap No; Lee, Chang Kyu
Title: Human Bocavirus in Patients with Respiratory Tract Infection Document date: 2011_6_28
ID: vuvgvz4n_7
Snippet: To obtain standard curves for absolute quantification, the PCR product of HBoV was cloned into a plasmid vector pGEM®-T Easy Vector (Promega, Madison, WI, USA), purified using a QIAprep mini prep kit (Qiagen Inc., Valencia, CA, USA), and quantified using UV spectroscopy (1 genomic copy = 5 × 10 -12 µg). Serial 10-fold dilutions of the cloned plasmid were prepared to generate the standard curves......
Document: To obtain standard curves for absolute quantification, the PCR product of HBoV was cloned into a plasmid vector pGEM®-T Easy Vector (Promega, Madison, WI, USA), purified using a QIAprep mini prep kit (Qiagen Inc., Valencia, CA, USA), and quantified using UV spectroscopy (1 genomic copy = 5 × 10 -12 µg). Serial 10-fold dilutions of the cloned plasmid were prepared to generate the standard curves.
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