Author: Alsuheel, Ali Mohammed; Ali, Abdelwahid Saeed; Al-Hakami, Ahmed Musa; Shati, Ayed Abdullah; Chandramoorthy, Harish C.; Al-Qahtani, Saleh Mohammed
Title: Human metapneumovirus in Pediatric Patients with Acute Respiratory Tract Infections in the Aseer Region of Saudi Arabia Document date: 2019_4_12
ID: xogzl1lv_11
Snippet: The cell suspension was washed several times with PBS and the final cell deposit was resuspended in 2 mL PBS (pH 7.5). The cells were then gently agitated by pipetting up and down until the cellular material was released from the mucus. Additional PBS was added until a smooth suspension was obtained, and any visible flecks of mucus were removed. After the cell separation process was completed, the obtained cell suspension was centrifuged at room .....
Document: The cell suspension was washed several times with PBS and the final cell deposit was resuspended in 2 mL PBS (pH 7.5). The cells were then gently agitated by pipetting up and down until the cellular material was released from the mucus. Additional PBS was added until a smooth suspension was obtained, and any visible flecks of mucus were removed. After the cell separation process was completed, the obtained cell suspension was centrifuged at room temperature (15°C-30°C) for 10 min at 3500 rpm and the supernatant was discarded. The final cell deposit was resuspended in PBS to dilute any remaining mucus and maintain high cell density.
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