Author: Jobling, Michael G.; Yang, ZhiJie; Kam, Wendy R.; Lencer, Wayne I.; Holmes, Randall K.
Title: A Single Native Ganglioside GM(1)-Binding Site Is Sufficient for Cholera Toxin To Bind to Cells and Complete the Intoxication Pathway Document date: 2012_10_30
ID: sxdstw4a_16
Snippet: One way that multivalent binding could affect CT function would be by enhancing the binding avidity for cell membranes containing GM 1 . In this and in our previous studies of the CT binding site mutants, we observe a loss of avidity for binding GM 1 when the GM 1 binding pockets are mutated. In the current studies, the loss of apparent avidity for the one-binding-site species is 10-fold, but the toxin still binds to GM 1 applied to the well at n.....
Document: One way that multivalent binding could affect CT function would be by enhancing the binding avidity for cell membranes containing GM 1 . In this and in our previous studies of the CT binding site mutants, we observe a loss of avidity for binding GM 1 when the GM 1 binding pockets are mutated. In the current studies, the loss of apparent avidity for the one-binding-site species is 10-fold, but the toxin still binds to GM 1 applied to the well at nanomolar concentrations, suggesting this may not fully explain the strong loss of toxin function.
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