Author: Girardi, Erika; Chane-Woon-Ming, Béatrice; Messmer, Mélanie; Kaukinen, Pasi; Pfeffer, Sébastien
Title: Identification of RNase L-Dependent, 3'-End-Modified, Viral Small RNAs in Sindbis Virus-Infected Mammalian Cells Document date: 2013_11_19
ID: v6uc0ijw_14
Snippet: In order to extend this observation and assess the role of RNase L in SvsRNA generation, we also produced small RNA libraries from HEK293 cells treated with a control or an RNase L-specific siRNA prior to SINV infection. In accordance with the striking effect seen on the three specific SvsRNAs, reduction in RNase L protein level decreased the entire population of clonable viral sequences from 2.75 to 1.19% (see Fig. S5A in the supplemental materi.....
Document: In order to extend this observation and assess the role of RNase L in SvsRNA generation, we also produced small RNA libraries from HEK293 cells treated with a control or an RNase L-specific siRNA prior to SINV infection. In accordance with the striking effect seen on the three specific SvsRNAs, reduction in RNase L protein level decreased the entire population of clonable viral sequences from 2.75 to 1.19% (see Fig. S5A in the supplemental material). However, RNase L knockdown affected neither the size distribution of viral reads nor their genomic distribution ( Fig. S5B and C).
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