Author: Lippens, Carla; Duraes, Fernanda V.; Dubrot, Juan; Brighouse, Dale; Lacroix, Mathilde; Irla, Magali; Aubry-Lachainaye, Jean-Pierre; Reith, Walter; Mandl, Judith N.; Hugues, Stéphanie
Title: IDO-orchestrated crosstalk between pDCs and Tregs inhibits autoimmunity Document date: 2016_12_23
ID: sl8148ap_18
Snippet: Treg cells were isolated from total skin LNs of naïve mice or from dLNs of EAE mice (day 10 after immunization). LNs were scratched and LN cells were subjected to CD4+ T cell enrichment using CD4+ T cell isolation kit (Miltenyi biotec). CD4+ CD25hi Treg cells were next sorted using a MoFlowAstrios (Beckman Coulter)......
Document: Treg cells were isolated from total skin LNs of naïve mice or from dLNs of EAE mice (day 10 after immunization). LNs were scratched and LN cells were subjected to CD4+ T cell enrichment using CD4+ T cell isolation kit (Miltenyi biotec). CD4+ CD25hi Treg cells were next sorted using a MoFlowAstrios (Beckman Coulter).
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