Author: Lippens, Carla; Duraes, Fernanda V.; Dubrot, Juan; Brighouse, Dale; Lacroix, Mathilde; Irla, Magali; Aubry-Lachainaye, Jean-Pierre; Reith, Walter; Mandl, Judith N.; Hugues, Stéphanie
Title: IDO-orchestrated crosstalk between pDCs and Tregs inhibits autoimmunity Document date: 2016_12_23
ID: sl8148ap_25
Snippet: WT and Rag2−/− pDCs were recovered from total skin LN and spleen after digestion with the enzymatic mix described above. pDCs were isolated using the Plasmacytoid Dendritic Cell isolation kit II (Miltenyi Biotec) according to manufacturer's instructions. pDCs were loaded or not with MOG35–55 peptide (10 μg/mL). 300 000 pDCs were seeded in 48 well plates with 200 000 2D2 CD4+ T cells. Cells were co-cultured in complete RPMI medium for 16 h .....
Document: WT and Rag2−/− pDCs were recovered from total skin LN and spleen after digestion with the enzymatic mix described above. pDCs were isolated using the Plasmacytoid Dendritic Cell isolation kit II (Miltenyi Biotec) according to manufacturer's instructions. pDCs were loaded or not with MOG35–55 peptide (10 μg/mL). 300 000 pDCs were seeded in 48 well plates with 200 000 2D2 CD4+ T cells. Cells were co-cultured in complete RPMI medium for 16 h and pDCs were isolated again using the Plasmacytoid Dendritic Cell isolation kit II (Myltenyi Biotech) according to manufacturer's instructions.
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