Author: Jo, Seri; Kim, Suwon; Shin, Dong Hae; Kim, Mi-Sun
Title: Inhibition of SARS-CoV 3CL protease by flavonoids Document date: 2019_11_14
ID: vynk8q8c_6
Snippet: The protein was purified by cation chromatography using a 5 ml Hi-Trap SP column (GE Healthcare, Piscataway, New Jersey, USA). The column was equilibrated with a buffer consisting of 50 mM MES pH 6.5 and the pooled fractions were loaded. The column was eluted using a linear NaCl gradient to 1 M NaCl and the protein was eluted at 0.23 M NaCl. SDS-PAGE showed one band around 22 kDa (21895.09 Da), corresponding to the molecular weight of the catalyt.....
Document: The protein was purified by cation chromatography using a 5 ml Hi-Trap SP column (GE Healthcare, Piscataway, New Jersey, USA). The column was equilibrated with a buffer consisting of 50 mM MES pH 6.5 and the pooled fractions were loaded. The column was eluted using a linear NaCl gradient to 1 M NaCl and the protein was eluted at 0.23 M NaCl. SDS-PAGE showed one band around 22 kDa (21895.09 Da), corresponding to the molecular weight of the catalytic domain of SARS-CoV 3CLpro. The protein was concentrated to 16 mg ml À1 for the protease assay in a buffer consisting of 0.23 M NaCl and 50 mM MES pH 6.5.
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