Author: Lippens, Carla; Duraes, Fernanda V.; Dubrot, Juan; Brighouse, Dale; Lacroix, Mathilde; Irla, Magali; Aubry-Lachainaye, Jean-Pierre; Reith, Walter; Mandl, Judith N.; Hugues, Stéphanie
Title: IDO-orchestrated crosstalk between pDCs and Tregs inhibits autoimmunity Document date: 2016_12_23
ID: sl8148ap_32
Snippet: Total RNA was isolated and prepared with TRIzol reagent (Invitrogen) and RT-PCR were performed as described [23]. cDNA was synthesized with random hexamers and M-MLV Reverse Transcriptase (Promega). PCR were performed with CFX Connect Real-time System (Bio rad) and iQ SYBR green Super-mix (Bio-Rad Labolatories). GAPDH mRNA was used for normalization. Primer sequences were as follows: IDO, forward, 5′-GGG ATG ACG ATG TTC GAA AG-3′ and reverse .....
Document: Total RNA was isolated and prepared with TRIzol reagent (Invitrogen) and RT-PCR were performed as described [23]. cDNA was synthesized with random hexamers and M-MLV Reverse Transcriptase (Promega). PCR were performed with CFX Connect Real-time System (Bio rad) and iQ SYBR green Super-mix (Bio-Rad Labolatories). GAPDH mRNA was used for normalization. Primer sequences were as follows: IDO, forward, 5′-GGG ATG ACG ATG TTC GAA AG-3′ and reverse 5′-CAG GAC ACA GTC TGC ATA AG-3′; GAPDH, forward, 5′- CCC GTA GAC AAA ATG GTG AAG -3′ and reverse 5′- AGG TCA ATG AAG GGG TCG TTG -3′.
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