Author: Rathore, Shailendra S.; Liu, Yinghui; Yu, Haijia; Wan, Chun; Lee, MyeongSeon; Yin, Qian; Stowell, Michael H.B.; Shen, Jingshi
Title: Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE Document date: 2019_12_24
ID: pudp1eoo_41
Snippet: Negative staining electron microscopy-Electron microscopy imaging of liposomes was carried out at Boulder Lab for 3D Electron Microscopy. Protein-free liposomes were incubated with buffer or a SNARE-derived peptide (added to a final concentration of 356 μM) for one hour at room temperature. The samples were then stained with 2% uranyl acetate and observed on a Philips CM100 scanning transmission electron microscope operated at 80 kV......
Document: Negative staining electron microscopy-Electron microscopy imaging of liposomes was carried out at Boulder Lab for 3D Electron Microscopy. Protein-free liposomes were incubated with buffer or a SNARE-derived peptide (added to a final concentration of 356 μM) for one hour at room temperature. The samples were then stained with 2% uranyl acetate and observed on a Philips CM100 scanning transmission electron microscope operated at 80 kV.
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